![]() It is useful for pinpointing data points that may be difficult to find using the tooltip popup box. Note that this tool locates the position of the mouse regardless of the spectra. For iPhone and iPad users, simply press on the location of the curve to view the same data display.You can also pinpoint your mouse position using the Mouse Position indicator below the selection area. The popup displays the fluorochrome, excitation or emission, the wavelength of light, and the % of maximum excitation or emission at that wavelength. To view exact data points on any graph, make sure the "Enable tooltip" box is selected and then mouse over spectra curves until a popup appears. Turn your filters on and off using the checkboxes. If you wish to visualize the degree of fluorescence transmission through your filter sets, enter the filter wavelength and bandpass into column 4. To visualize laser lines select your desired line from the listed options Excitation spectra are displayed as dotted lines, while emission spectra are displayed as smooth lines. The graph legend appears above the display, indicating the displayed spectrum. To view spectra, simply select a fluorophore from one of the dropdowns, then select or deselect the emission and/or excitation spectrum of your fluorochrome of interest. To view the spectral signatures of fluorochromes in a spectral flow cytometer, please visit the Aurora Spectra Analyzer. Spark Blue™ 550, Spark NIR™ 685) are only recommended for use in multicolor spectral flow cytometry panels. Line spectra full#To view a full listing of Trademarks and Patents Click Here. This page works best in IE9 or higher, Firefox, or Chrome.įeel free to copy displays that you generate here using the print screen function on your computer. BioLegend's Fluorescence Spectra Analyzer is useful for the analysis of excitation and emission spectra of commonly used fluorochromes for flow cytometry. ![]()
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